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Journal: Biomedical Optics Express
Article Title: High-speed wide-field fluorescence lifetime imaging for intraoperative tumor visualization and in vivo multiplexing
doi: 10.1364/BOE.568712
Figure Lengend Snippet: ( a) Schematic of the TD imaging system for S-FLT acquisition, comprising of a gated intensifier connected to a CMOS camera, and a CW camera attached to the intensifier via a beam splitter (BS) for parallel acquisition of CW and time gated images. A pulsed laser source (EXR-20, SuperK Varia, NKT Photonics, 400–850 nm tuning range, 80 MHz repetition rate, < 20 ps pulse width, or FFSMART, Toptica, 780 nm, 78 MHz repetition rate, 120 fs pulse width) coupled to a multimode optical fiber illuminates the sample (S) via a light diffuser (D). Fluorescence is collected using a macro lens coupled with a filter wheel (F) with multiple bandpass filters ( Supplement 1 Table S1). (b) Schematic of the proposed S-FLT method: Typical TD fluorescence decay curve (in log scale) shown with the choice of the single time gate “ T ” used in the S-FLT calculation (Eq. ), which is an explicit formula for the lifetime τ in terms of the single gate measurement I ( T ) and the parallel CW measurement I C W . (c) Mean square error and variance of FLTs estimated from RLD (left) and S-FLT (right), as a function of SNR and true FLT. (See Supplement 1 Section 2.A for details regarding the simulation and a plot of noise performance for a wider range of SNRs).
Article Snippet: A
Techniques: Imaging, Fluorescence